Contact an Account ManagerSensitive HPLC determination of 6-mercaptopurine 6-thioguanine 6-mercaptopurine riboside and 6-thioguanosine in biological fluids
Summary
Type: HPLC
Application #: 107140
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 312 and 342nm as indicated
Conditions: eluent A: 0.025M phosphoric acid pH 2.75, eluent B: 50% methanol (v/v), eluent C: 0.10M KH2PO4 pH 6.6, gradient sequence: starting with 100% A, ratio A:B:C (v/v) after 5min 98:2:0, after 10min 30:3.5:66.5, then isocratic at this composition
flow rate 1.7ml/min
temperature 33°C
Authors: van Baal JM
Source: J Chromatogr 336 (1984) 422 - 428
Details
Legend: Fig. 1. Separation of 6-thiopurines,
A) blank plasma, CSF and urine samples
B) plasma, CSF and urine samples spiked with
1.) 6-thiouric acid
2.) 6-thioxanthine
3.) 0.7µM 6-thioguanine
4.) 0.4µ